骨橋素(OPN)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- 編號USCA899Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長2h, 40min
- 檢測范圍6.86-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于2.47pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1814 ¥ 2592 ¥ 11664 ¥ 22032 ¥ 181440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測骨橋素(OPN)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的骨橋素(OPN)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-92 | 87 |
| EDTA plasma(n=5) | 90-101 | 97 |
| heparin plasma(n=5) | 80-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的骨橋素(OPN)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中骨橋素(OPN)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-89% | 95-102% | 99-105% | 81-92% |
| EDTA plasma(n=5) | 81-90% | 81-101% | 90-104% | 94-101% |
| heparin plasma(n=5) | 89-97% | 94-101% | 95-102% | 90-97% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實(shí)驗(yàn)原理
將骨橋素(OPN)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的骨橋素(OPN)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的骨橋素(OPN)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的骨橋素(OPN)呈正相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA899Mu01 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA899Mu61 | 骨橋素(OPN)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA899Mu03 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA899Mu61 | 骨橋素(OPN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA899Mu02 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA899Mu01 | 骨橋素(OPN)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA899Mu02 | 骨橋素(OPN)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA899Mu06 | 骨橋素(OPN)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA899Mu | 骨橋素(OPN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA899Mu | 骨橋素(OPN)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA899Mu | 骨橋素(OPN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA899Mu01 | 骨橋素(OPN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Biomedical Materials Research Part A | Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture [PubMed: 19280635] |
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| The Journal of Rheumatology | Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source] |
| International Journal of Pharmaceutics | Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742] |
| European Cells and matreials | The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source] |
| Journal of Equine Veterinary Science | Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source] |
| Basic & Clinical Pharmacology & Toxicology | Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source] |
| Journal of Cellular Biochemistry | Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337] |
| FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352] |
| Gene | Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150] |
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| Int J Biol Sci | Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817] |
| Journal of Kunming Medical University / Kunming Yike Daxue Xuebao | Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost] |
| Am J Pathol.? | Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343] |
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| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817] |
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| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824] |
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| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells [] | |
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