補(bǔ)體因子B(CFB)活性蛋白
Active Complement Factor B (CFB)
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
- 編號(hào)UAPC011Hu03
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Complement Factor B (CFB) a component of the alternative pathway of complement activation. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. The method of functional assay of CFB was tested in hemolysis assays. Two-fold dilute the recombinant human CFB with 0.9% NaCl, 2mmol/L MgCl2 ,and then add same volume of 1% rabbit erythrocyte (RaE) in 8mmol/L EDTA, the negative control only without MgCl2. All the samples incubated at 37℃. After 3 hours later, take 10μL supernatant and 90μL TMB incubated at 37℃ for 5-10 minutes. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The results are shown in Figure 1. It was obvious that the minimal effective concentration of CFB is 0.3125μg/mL.(A) 1% rabbit erythrocyte (RaE) treated with 0.3125μg/mL CFB for 1h
(B) Negative control (1% RaE treated with 0.3125ug/mL CFB, 8mmol/L EDTA) without MgCl2.
Figure. The hemolysis effection of recombinant human CFB
Figure. The hemolysis activity of recombinant human CFB
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC011Hu05 | 補(bǔ)體因子B(CFB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC011Hu03 | 補(bǔ)體因子B(CFB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC011Hu02 | 補(bǔ)體因子B(CFB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPC011Hu61 | 補(bǔ)體因子B(CFB)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC011Hu04 | 補(bǔ)體因子B(CFB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC011Hu01 | 補(bǔ)體因子B(CFB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC011Hu03 | 補(bǔ)體因子B(CFB)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAC011Hu03 | 補(bǔ)體因子B(CFB)多克隆抗體 | WB; IHC |
| UPAC011Hu02 | 補(bǔ)體因子B(CFB)多克隆抗體 | WB; IHC |
| UPAC011Hu01 | 補(bǔ)體因子B(CFB)多克隆抗體 | WB; IHC |
| UPAC011Hu04 | 補(bǔ)體因子B(CFB)多克隆抗體 | WB; IHC |
| ULAC011Hu71 | 補(bǔ)體因子B(CFB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAC011Hu22 | 補(bǔ)體因子B(CFB)單克隆抗體 | WB; IHC; ICC; IP. |
| UWEC011Hu | 補(bǔ)體因子B(CFB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEC011Hu | 補(bǔ)體因子B(CFB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC011Hu | 補(bǔ)體因子B(CFB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BMC Nephrology | Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis [BioMed: 14712369] |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z] |
| Pediatr Int | Atypical hemolytic uremic syndrome: Korean pediatric series [PubMed: 25443527] |
| Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358] |
| Biochemical and Biophysical Research Communications | Adipose tissue complement factor B promotes adipocyte maturation. [pubmed:29137982] |
| Journal?of?proteome?research | Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection [] |
| journal of human genetics | Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population [Pubmed: 31857673] |
| Nephrol Dial Transplant | Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis [33367879] |
| JOURNAL OF GENERAL VIROLOGY | Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse [33410734] |
| J Proteome Res | Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role [34766501] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |
