UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)活性蛋白
Active UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1)
UGT1; GNT1; UGT1A; UDPGT; HUG-BR1; UGT1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-1
- 編號(hào)UAPG920Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點(diǎn)6.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
UDP-Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1)，as known as GNT1 or UGT1，is a member of the UDP-glucuronosyltransferase (UGT) enzyme family, which plays a critical role in the metabolism of various compounds in the body. This enzyme catalyzes the conjugation of bilirubin, steroids, and other lipophilic compounds with glucuronic acid, facilitating their excretion from the body.The binding of UGT1A1 and Cytochrome P450 1A1 (CYP1A1) is particularly importantin the metabolism of drugs and xenobiotics, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human UGT1A1 and recombinant rat CYP1A1. Briefly, biotin-linked UGT1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to CYP1A1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of UGT1A1 and CYP1A1 was shown in Figure 1, the EC50?for this effect is 0.08ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPG920Hu01 | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPG920Hu01 | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAG920Hu01 | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)多克隆抗體 | WB; IHC |
| UMAG920Hu21 | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEG920Hu | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMG920Hu | UDP葡糖醛酸基轉(zhuǎn)移酶1家族多肽A1(UGT1A1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats [Pubmed:25733458] |
| The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,5 [PubMed: 25733458] |
| Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590] |
| Molecular Nutrition &?Food?Research | Phenethyl isothiocyanate?and?indole-3-carbinol?from?cruciferous?vegetables, but not furanocoumarins from apiaceous?vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590] |
| Journal of Medicinal Chemistry | A practical and high-affinity ?uorescent probe for uridine diphosphate glucuronosyltransferase 1A1: a good surrogate for bilirubin [pubmed:29125289] |
| International Journal of Nanomedicine | Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions [Pubmed: 32764932] |
