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泛素羧基端酯酶L1(UCHL1)多克隆抗體

DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat UCHL1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Rat Pancreas Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Rat UCHL1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat UCHL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Pancreas Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat UCHL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant UCHL1, Rat.
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: UCHL1 knockout A549 cell lysate;
Predicted MW: 25kd
Observed MW: 26kd
Primary Ab: 1μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Spinal cord lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Testis lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: SH-SY5Y cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Rat UCHL1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對UCHL1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別UCHL1。

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

雜志	參考文獻
J Neurol Sci	Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084]
Journal of the Neurological Sciences	Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533]
Ulutas Medical Journal	Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135]
Scientific Reports	Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290]
Intensive?Care?Medicine	Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900]
Earth and Environmental Science	Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088]
	Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9]
Journal of Critical Care	Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448]
Brain Sciences	BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792]

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編號	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPG945Ra02	泛素羧基端酯酶L1(UCHL1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPG945Ra01	泛素羧基端酯酶L1(UCHL1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAG945Ra01	泛素羧基端酯酶L1(UCHL1)多克隆抗體	WB; IHC
ULAG945Ra71	泛素羧基端酯酶L1(UCHL1)多克隆抗體(生物素標記)	WB
ULAG945Ra81	泛素羧基端酯酶L1(UCHL1)多克隆抗體(異硫氰酸熒光素標記)	WB; IHC; ICC; IF.
UMAG945Ra21	泛素羧基端酯酶L1(UCHL1)單克隆抗體	WB; IHC; ICC; IP.
USEG945Ra	泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCG945Ra	泛素羧基端酯酶L1(UCHL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMG945Ra	泛素羧基端酯酶L1(UCHL1)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSG945Ra01	泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".