鐵調素(Hepc)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Hepcidin (Hepc)
HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
- 編號USCB979Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍5.49-4,000pg/mL
- 靈敏度最小可檢測劑量小于等于1.89pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1764 ¥ 2520 ¥ 11340 ¥ 21420 ¥ 176400
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測鐵調素(Hepc)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的鐵調素(Hepc)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-99 | 93 |
| EDTA plasma(n=5) | 78-101 | 91 |
| heparin plasma(n=5) | 82-102 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的鐵調素(Hepc)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中鐵調素(Hepc)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-103% | 80-103% | 79-102% | 85-93% |
| EDTA plasma(n=5) | 87-95% | 83-104% | 86-99% | 92-104% |
| heparin plasma(n=5) | 88-102% | 78-95% | 97-104% | 82-96% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將鐵調素(Hepc)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的鐵調素(Hepc)與連接于固相載體上的抗體結合，然后加入生物素化的鐵調素(Hepc)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發(fā)射光強度和樣品中的鐵調素(Hepc)呈正相關。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UEPB979Hu61 | 鐵調素(Hepc)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB979Hu01 | 鐵調素(Hepc)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB979Hu61 | 鐵調素(Hepc)活性蛋白 | Cell?culture;?Activity?Assays. |
| UCPB979Hu12 | 鐵調素(Hepc)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu22 | 鐵調素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu23 | 鐵調素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu21 | 鐵調素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAB979Hu07 | 鐵調素(Hepc)多克隆抗體 | IHC |
| UPAB979Hu09 | 鐵調素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB979Hu06 | 鐵調素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB979Hu01 | 鐵調素(Hepc)多克隆抗體 | IHC |
| UPAB979Hu08 | 鐵調素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAB979Hu81 | 鐵調素(Hepc)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAB979Hu71 | 鐵調素(Hepc)多克隆抗體(生物素標記) | IHC |
| UMAB979Hu26 | 鐵調素(Hepc)單克隆抗體 | Testing in progress |
| UMAB979Hu22 | 鐵調素(Hepc)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAB979Hu21 | 鐵調素(Hepc)單克隆抗體 | IHC |
| URAB979Hu21 | 鐵調素(Hepc)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| USEB979Hu | 鐵調素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEB979Hu | 鐵調素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| UCCB979Hu | 鐵調素(Hepc)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB979Hu | 鐵調素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB979Hu11 | 鐵調素(Hepc)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Tuberculosis | Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity [10.1016/j.tube.2017.11.011] |
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| Frontiers in Physiology | Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers [Pubmed: 32038278] |
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