骨鈣素(OC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Osteocalcin (OC)
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
- 編號(hào)UKSA471Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.625-40ng/mL
- 可適靈敏度0.252ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)骨鈣素(OC)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA471Hu01 | 骨鈣素(OC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA471Hu02 | 骨鈣素(OC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA471Hu21 | 骨鈣素(OC)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA471Hu22 | 骨鈣素(OC)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA471Hu23 | 骨鈣素(OC)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA471Hu31 | 骨鈣素(OC)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA471Hu02 | 骨鈣素(OC)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA471Hu01 | 骨鈣素(OC)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA471Hu08 | 骨鈣素(OC)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA471Hu24 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu25 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu27 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu29 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu26 | 骨鈣素(OC)單克隆抗體 | ICC/IF |
| UMAA471Hu28 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu21 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu23 | 骨鈣素(OC)單克隆抗體 | IHC |
| UMAA471Hu22 | 骨鈣素(OC)單克隆抗體 | IHC |
| USEA471Hu | 骨鈣素(OC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA471Hu | 骨鈣素(OC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA471Hu | 骨鈣素(OC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA471Hu01 | 骨鈣素(OC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| Pharmacology | MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal [Pubmed: 31694033] |
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| Biomedicine & Pharmacotherapy | Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone loss [Pubmed: 31846839] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
| Theranostics | EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis [Pubmed: 32194868] |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy [Pubmed: 32172552] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells [Pubmed: 32064734] |
| CELL BIOCHEMISTRY AND FUNCTION | HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinase [Pubmed: 32567086] |
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| Food & Function | Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the?… [Pubmed: 33140795] |
| ADVANCED FUNCTIONAL MATERIALS | A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration [] |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene [] |
| 淫羊藿水提物及提取藥渣的抗骨質(zhì)疏松藥效比較研究 [] | |
| Chemical Engineering Journal | Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances [] |
| Surfaces and Interfaces | In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching [] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/|??\catenin pathway [33950533] |
| Biochemical Engineering Journal | Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate [] |
| Br J Pharmacol | Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis [34233019] |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism [34511043] |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780] |
| ACS Appl Mater Interfaces | Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic Nanomedicine [Pubmed:35311248] |
| Molecules | Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells [Pubmed:35684342] |
| Journal of Exercise Science & Fitness | Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young Females [Pubmed:35096081] |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing [] |
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