神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Neuropeptide Y (NPY)
NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY
- 編號UCEA879Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24.69-2000pg/mL
- 靈敏度最小可檢測劑量小于等于9.35pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1588 ¥ 2268 ¥ 10206 ¥ 19278 ¥ 158760
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測神經(jīng)肽Y(NPY)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的神經(jīng)肽Y(NPY)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 102 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 87-101 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)肽Y(NPY)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)肽Y(NPY)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 84-101% | 88-96% | 93-105% |
| EDTA plasma(n=5) | 94-102% | 95-104% | 89-103% | 88-102% |
| heparin plasma(n=5) | 95-105% | 87-94% | 82-103% | 78-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將神經(jīng)肽Y(NPY)單克隆抗體包被微孔板，制成固相載 體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA879Bo01 | 神經(jīng)肽Y(NPY)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA879Bo01 | 神經(jīng)肽Y(NPY)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA879Bo21 | 神經(jīng)肽Y(NPY)單克隆抗體 | WB; IHC; ICC; IP. |
| UCEA879Bo | 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA879Bo | 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Hemodialysis International | Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease [Onlinelibrary: hdi.12072] |
| Journal of Pediatric Endocrinology and Metabolism | Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children [Degruyter: Source] |
| Ren Fail. | The role of adipocytokines on depressive symptoms of patients with chronic kidney disease [Pubmed: 23879728] |
| Coronary Artery Disease | The relationship of plasma neuropeptide Y levels with coronary collateral development. [Pubmed: 24077326] |
| Coronary Artery Disease | The relationship of plasma neuropeptide Y levels with coronary collateral development [Lww:Source] |
| Toxicology Letters | Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392] |
| J Neurosurg Anesthesiol | High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage [PubMed: 25955865] |
| Journal of Diabetes Research | High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … [Pubmed:26885531] |
| Archives of Gynecology and Obstetrics | Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate [pubmed:27604241] |
| basic & clinical pharmacology & toxicology | Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. [pubmed:27625336] |
| Acta?Neurobiologiae Experimentalis | Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat [pubmed:27685769] |
| Cardiovascular?research | Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis [Pubmed:29850786] |
| Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594] |
| Neuropeptides | Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637] |
| Pharmacology | Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y?… [Pubmed: 30227410] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome [] |
| Marmara Medical Journal | Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome. [] |
| neurochemical research | Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. [Pubmed: 32378074] |
| Hum Psychopharmacol | Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double?\blind randomized??- [33245168] |
| Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100] |
