生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Growth Hormone (GH)
GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
- 編號UCEA044Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍123.5-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于49.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測生長激素(GH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的生長激素(GH)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 87-101 | 97 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 86-101 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的生長激素(GH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中生長激素(GH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 87-102% | 84-95% | 99-105% |
| EDTA plasma(n=5) | 96-103% | 80-98% | 93-101% | 88-102% |
| heparin plasma(n=5) | 81-101% | 87-95% | 86-101% | 78-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將生長激素(GH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA044Ca61 | 生長激素(GH)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA044Ca61 | 生長激素(GH)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA044Ca01 | 生長激素(GH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA044Ca01 | 生長激素(GH)多克隆抗體 | WB |
| UMAA044Ca21 | 生長激素(GH)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA044Ca | 生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA044Ca | 生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA044Ca | 生長激素(GH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPGA044Ca01 | 生長激素(GH)引物對 | PCR |
參考文獻
| 雜志 | 參考文獻 |
| Neuroscience Letters | The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats [PubMed: 19765635] |
| Cellular Reprogramming | Changes in the Somatotrophic Axis in Genetically Identical Dogs [PubMed: 21740269] |
| Clinical Cancer Research | The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status [PubMed: 21642381] |
| General and Comparative Endocrinology | Growth hormone expression and neuroprotective activity in a quail neural retina cell line [ScienceDirect: S0016648009002457] |
| Nutrition and Metabolism | Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis [Nutrition&Metabolism: source] |
| Veterinary Recprd Case Report | Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism [Bmj: Source] |
| Journal of the American Animal Hospital Association | GH Deficiency in a Dalmatian Puppy with Megaesophagus [Pubmed: 24051259] |
| Biotechnology and Bioprocess Engineering | Functional expression of bovine growth hormone gene in Pleurotus eryngii [Springer: Source] |
| Buletin Veteriner Udayana | Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali [Unud:Source] |
| Veterinary World | Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows [Veterinaryworld:Source] |
| Int J Clin Exp Med | Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831] |
| Acta Histochemica | Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666] |
| ANNALS OF ANIMAL SCIENCE | Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87] |
| Annals of Animal Science | Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87] |
| Journal?of?Dairy?Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503] |
| Jurnal Riset Akuakultur | TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA [1329] |
| Trop Anim Health Prod | Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes [pubmed:28785907] |
| Biological Trace Element Research | Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007] |
| Revista Brasileira de Ciência Avícola | Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285] |
| 畜牧獸醫(yī)學(xué)報 | 單色綠光對雞胚褪黑激素合成的影響 [DOI: 10.11843/j.issn.0366-6964.2017.05.019] |
| Obesity Surgery | Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice [pubmed:28853013] |
| JURNAL VETERINER | Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) [10.19087/jveteriner.2017.18.2.226] |
| Veterinary World. | Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. [pubmed:29184372] |
| Biochemistry, Genetics and Molecular Biology | Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs [10.5772/intechopen.68545] |
| Cellular Physiology and Biochemistry | Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease Model [Pubmed:29975946] |
| PLoS One | Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588] |
| Pakistan Journal of Zoology | Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. [:] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
| International Journal of Current Microbiology and Applied Sciences | Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons [] |
| Photochemistry and Photobiology | Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of Chicks [Pubmed: 31111492] |
| Journal of Animal Science | Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigs [Pubmed: 31922564] |
| SAUDI MEDICAL JOURNAL | The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive [Pubmed: 31287125] |
| Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
| JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | Isolated growth hormone deficiency in a Chihuahua with a GH1 mutation [Pubmed: 32646299] |
| Scientific Reports | Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs [33244103] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling [33492754] |
| animals | Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes [34359233] |
