組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay)
Histamin
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)ULMA927Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍0.1-100ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.033 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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特異性
本試劑盒用于檢測(cè)組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-95 | 89 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 90-97 | 94 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-104% | 93-102% | 82-95% | 81-103% |
| EDTA plasma(n=5) | 80-101% | 97-105% | 89-97% | 79-94% |
| heparin plasma(n=5) | 79-89% | 81-101% | 80-105% | 85-99% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測(cè)溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
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| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA927Ge41 | 組胺(HA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA927Ge11 | 組胺(HA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA927Ge21 | 組胺(HA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA927Ge01 | 組胺(HA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA927Ge71 | 組胺(HA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA927Ge81 | 組胺(HA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA927Ge21 | 組胺(HA)單克隆抗體 | IHC |
| UCEA927Ge | 組胺(HA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA927Ge | 組胺(HA)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA927Ge | 組胺(HA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA927Ge11 | 組胺(HA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Allergy & Clinical Immunology | PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice [Pubmed: 21981059] |
| Asian Pacific Journal of Allergy and Immunology | Comparison of sensitization between β-lactoglobulin and its hydrolysates [Digital: 875] |
| J Chromatogr A | Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection [PubMed: 26372445] |
| Neurogastroenterology & Motility | Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641] |
| Italian Journal of Animal Science | Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488] |
| Int J Clin Exp Pathol | Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf] |
| PLoS One. | Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762] |
| Int Immunopharmacol. | Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal?Society?Open?Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| International?Journal?of?Pharmaceutical?Sciences?and?Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| Allergy | Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Nature Communications | A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752] |
| CCR3?shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345] | |
| ANALYTICA CHIMICA ACTA | Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of?… [Pubmed: 32892931] |
| Annals of Tropical Medicine & Public Health | Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq [] |
| pharmacol res& perspect | Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs [34014033] |
