甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Parathyroid Hormone (PTH) ,etc. by FLIA (Flow Luminescence Immunoassay)
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA866Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.78-800pg/mL
- 靈敏度最小可檢測劑量小于等于0.26 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-104 | 99 |
| EDTA plasma(n=5) | 80-92 | 86 |
| heparin plasma(n=5) | 79-92 | 78 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-91% | 79-91% | 87-101% | 97-105% |
| EDTA plasma(n=5) | 78-101% | 78-89% | 97-105% | 98-105% |
| heparin plasma(n=5) | 92-101% | 93-101% | 82-101% | 87-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA866Ra01 | 甲狀旁腺激素(PTH)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA866Ra01 | 甲狀旁腺激素(PTH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA866Ra11 | 甲狀旁腺激素(PTH)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA866Ra21 | 甲狀旁腺激素(PTH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA866Ra08 | 甲狀旁腺激素(PTH)多克隆抗體 | WB |
| UPAA866Ra01 | 甲狀旁腺激素(PTH)多克隆抗體 | WB |
| UMAA866Ra21 | 甲狀旁腺激素(PTH)單克隆抗體 | WB; IHC; ICC; IP. |
| UCEA866Ra | 甲狀旁腺激素(PTH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA866Ra | 甲狀旁腺激素(PTH)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA866Ra | 甲狀旁腺激素(PTH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA866Ra11 | 甲狀旁腺激素(PTH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Biological trace element research | Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet [PubMed: 19915804] |
| Basic nutritional investigation | Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice [ScienceDirect: S0899900709004808] |
| The Korean Journal of Nutrition | Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats [KoreaMed: source] |
| Acta Physiologica Hungarica | Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters [Pubmed: 22173025] |
| Toxicology and Industrial Health | The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat [PubMed: 22782709] |
| Indian Journal of Experimental Biology | Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. [PubMed: 22439438] |
| Plos one | Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? [PubMed: PMC3679169] |
| Nutr Hosp. | Effect of the “protein diet” and bone tissue. [Pubmed: 24483972] |
| Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
| Nutricion Hospitalaria | Effect of the “protein diet” and bone tissue [Aulamedica: Source] |
| Academic Journal | Effect of whole body vibration on healthy rat plasma parameters [Ebscohost: Source] |
| J Proteome Res. | Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets [Pubmed:25051233] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
| J Nutr Sci Vitaminol (Tokyo) | The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats [PubMed: 26639847] |
| The Korean Society of Pharmacognosy | Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat [Article: Hksobf_2015_V46N2_167] |
| J?Bone?Miner Res | Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats [Pubmed:26175082] |
| 7 | Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. [pubmed:28218908] |
| PLOS?ONE | Prostaglandin-E2?Mediated?Increase?in?Calcium?and?Phosphate?Excretion?in a?Mouse?Model?ofDistal?Nephron?Salt?Wasting. [pubmed:27442254] |
| International Journal of Advanced Research in Biological Sciences | Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat [10.22192/ijarbs.2017.04.04.018] |
| American Journal of Physiology-Renal?Physiology | Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice [Pubmed:29363324] |
| Tropical?Animal?Health?and Production | Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism [Pubmed:29931604] |
| International Immunopharmacology | CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts [Pubmed: 31679847] |
| ENDOCRINOLOGY | A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats [Pubmed: 32852547] |
| Kidney International | Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts. [] |
| PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES [] | |
| Oxid Med Cell Longev | Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766] |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector [34599290] |
| Cells | Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury [Pubmed:35456008] |
| African Health Sciences | Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease [] |
