神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體
Monoclonal Antibody to Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- 編號UMAA537Hu24
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)A4
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA537Hu01-神經(jīng)元特異性烯醇化酶(NSE)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 324 ¥ 756 ¥ 1080 ¥ 2700 ¥ 10800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 10μg/ml Mouse Anti-Human NSE Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Rat Cerebrum lysate; Lane3: Porcine Cerebrum lysate Primary Ab: 2μg/ml Mouse Anti-Human NSE Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對NSE的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NSE。
用法
Western blottin:g: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA537Hu02 | 神經(jīng)元特異性烯醇化酶(NSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體 | WB; IHC |
| UPAA537Hu02 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體 | WB |
| ULAA537Hu71 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體(生物素標(biāo)記) | WB |
| ULAA537Hu81 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA537Hu27 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB |
| UMAA537Hu29 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu25 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu21 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu22 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu23 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu26 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu28 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu24 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| USEA537Hu | 神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA537Hu | 神經(jīng)元特異性烯醇化酶(NSE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UCGA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)cDNA | PCR、蛋白表達等 |
參考文獻
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| Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247] |
| Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349] |
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| Physiol Rep | S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156] |
| PLoS One | Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits [PubMed: 26645542] |
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| Journal of the Neurological?Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid?… [Pubmed:29801873] |
| Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| The?Eurasian?Journal?of?Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?… [] |
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| The role of light desynchronosis in the development of stress-induced aging [] | |
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| Cephalalgia | Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195] |
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